{"created":"2023-06-20T12:59:29.312835+00:00","id":1321,"links":{},"metadata":{"_buckets":{"deposit":"ff63b6a3-65b8-411f-8b01-3c3e72809d76"},"_deposit":{"created_by":17,"id":"1321","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"1321"},"status":"published"},"_oai":{"id":"oai:nufs-nuas.repo.nii.ac.jp:00001321","sets":["1:9:97:147"]},"author_link":["566"],"item_10002_biblio_info_7":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-12-25","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"58","bibliographicPageStart":"51","bibliographic_titles":[{"bibliographic_title":"名古屋栄養科学雑誌"},{"bibliographic_title":"Nagoya Journal of Nutritional Sciences","bibliographic_titleLang":"en"}]}]},"item_10002_description_5":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Brown adipocytes expressed a large variety of purinergic receptor subtypes. We investigated about the expression of P2X5 receptor subtype among purinergic receptor subtypes expressed in mouse adipose tissue.Ca2+ imaging showed that applied 10 μM ATP or 1 μM 2MeSATP (a P2X5, P2Y1 and P2Y11 agonist) increased intracellular Ca2+ concentration in brown adipocytes. RT-PCR and real-time PCR studies revealed that P2X5 receptor mRNA was expressed much in mouse brown adipocytes but less in white adipocytes. The expression levels of P2X5 receptor mRNA in brown adipocytes were about nine times higher than that of white adipocytes.Western blotting studies suggested the expression of P2X5 receptor protein was detected in brown adipocytes but not in white adipocytes. Immunohistostaining studies indicated the expression of P2X5 receptor protein was shown on brown adipocytes but not on fibroblasts. Thus, P2X5 receptors have a function for Ca2+ rises and it can be available as tools to identify and target brown adipocytes.","subitem_description_type":"Abstract"},{"subitem_description":"褐色脂肪細胞は熱産生器官である。寒冷曝露により交感神経から放出されたノルアドレナリンを受容し、中性脂肪から遊離脂肪酸を生成する。この遊離脂肪酸がもつエネルギーを熱に変換する事で熱産生を行っている。その際、褐色脂肪細胞内ではCa2+ 濃度が上昇する。このCa2+ は褐色脂肪細胞での熱産生量および酸素消費量を増大する事が知られている。交感神経はノルアドレナリンと同時にATP を神経伝達物質として放出する。\n昨年の私たちの研究により、マウス褐色脂肪細胞における複数のATP 受容体サブタイプ発現が確認された。その中でもP2X5受容体シグナルが遺伝子レベルにおいて強い事が示されたが、タンパクレベルでの発現の有無や、他の脂肪組織におけるP2X5受容体の存在については明らかにされていなかった。\n今回の研究により、マウス褐色脂肪細胞がP2X5受容体タンパクを機能的に発現する事が確認された。また脂肪組織間におけるP2X5遺伝子発現量を比較したところ、白色脂肪細胞に比べ褐色脂肪細胞では約9 倍発現量が高い事が示された。白色脂肪細胞および繊維芽細胞にはP2X5受容体タンパクは確認できなかった。以上の結果から、脂肪組織においてP2X5受容体の分布を調べる事で、褐色脂肪細胞の分布を比較的容易に確認する事ができると思われる。これらP2X5受容体は、交感神経から放出されたATP を受容する事で細胞内Ca2+ 濃度を上昇させ、熱産生を促進している可能性が考えられる。","subitem_description_type":"Abstract"}]},"item_10002_identifier_registration":{"attribute_name":"ID登録","attribute_value_mlt":[{"subitem_identifier_reg_text":"10.15073/00001255","subitem_identifier_reg_type":"JaLC"}]},"item_10002_publisher_8":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"名古屋学芸大学管理栄養学部"}]},"item_10002_relation_12":{"attribute_name":"論文ID（NAID）","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"40021472948","subitem_relation_type_select":"NAID"}}]},"item_10002_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"21892121","subitem_source_identifier_type":"ISSN"}]},"item_10002_title_1":{"attribute_name":"その他（別言語等）のタイトル","attribute_value_mlt":[{"subitem_title":"マウス褐色脂肪細胞マーカーとしてのP2X5受容体の有用性"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorAffiliations":[{"affiliationNameIdentifiers":[{"affiliationNameIdentifier":"","affiliationNameIdentifierScheme":"ISNI","affiliationNameIdentifierURI":"http://www.isni.org/isni/"}],"affiliationNames":[{"affiliationName":"","affiliationNameLang":"ja"}]}],"creatorNames":[{"creatorName":"早戸, 亮太郎","creatorNameLang":"ja"},{"creatorName":"ハヤト, リョウタロウ","creatorNameLang":"ja-Kana"},{"creatorName":"HAYATO, Ryotaro","creatorNameLang":"en"}],"familyNames":[{},{},{}],"givenNames":[{},{},{}],"nameIdentifiers":[{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-06-01"}],"displaytype":"detail","filename":"njns2-06.pdf","filesize":[{"value":"378.1 kB"}],"format":"application/pdf","licensetype":"license_11","mimetype":"application/pdf","url":{"label":"本文","url":"https://nufs-nuas.repo.nii.ac.jp/record/1321/files/njns2-06.pdf"},"version_id":"ea3437fb-1fdd-4ccb-9705-9ea3d43210b6"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"brown adipocytes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"white adipocyte","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"P2X₅","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"cell marker","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"P2X₅ receptor is functionally expressed on mouse brown adipocyte and can be a cell surface marker","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"P2X₅ receptor is functionally expressed on mouse brown adipocyte and can be a cell surface marker"}]},"item_type_id":"10002","owner":"17","path":["147"],"pubdate":{"attribute_name":"公開日","attribute_value":"2019-06-01"},"publish_date":"2019-06-01","publish_status":"0","recid":"1321","relation_version_is_last":true,"title":["P2X₅ receptor is functionally expressed on mouse brown adipocyte and can be a cell surface marker"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2023-11-13T05:32:44.661321+00:00"}